mechanism of drought stress tolerance of gf677 rootstock (peach and almond hybride) under in vitro conditions

نویسندگان

مهری مشایخی

دانشجوی کارشناسی ارشد گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران فریبرز حبیبی

کارشناس ارشد گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران محمد اسماعیل امیری

دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

چکیده

mechanisms of drought stress tolerance of gf677 rootstock, peach and almond hybrid, (prunus persica × prunus amygdalus) were studied under in vitro conditions. plantlets of gf677 rootstock were subcultured into the murashige and skoog (ms) solid proliferation medium containing 1 mg/l ba (6-benzyladenine) and 0.1 mg/l naa (naphthaline acetic acid) in four drought stress levels 0 (control), 10, 20 and 30 g/l polyethylene glycol 6000 (corresponding to osmotic potentials 0, -0.2, -0.4 and -0.6 mpa, respectively). after six weeks results showed that induced drought stress had a significant effect on measured parameters. by increasing drought levels in the culture medium, antioxidant enzymes activity (catalase and peroxidase), total protein content and proline content significantly increased; meanwhile soluble sugars had non-significant increasing in the different levels of drought. according to the results it can be concluded that, the most important mechanisms of drought tolerance of gf677 rootstock under in vitro conditions are the use of antioxidant defense system, increasing protein synthesis (enhancing genes expression) and proline accumulation and osmoregulation by soluble sugars had less important.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of Drought Tolerance in Safflower (Carthamus Tinctorius L.)Under non Stress and Drought Stress Conditions

This study was conducted to determine drought tolerance genotypes with superiority in different stressed environments. To screening quantitative indices of drought tolerance, genotypes of safflower (Carthamus tinctorius L.) were tested in a complete randomized block design with three replications under two different water regimes (irrigated and rainfed). Significant positive correlatio...

متن کامل

Evaluation of Drought Tolerance in Safflower (Carthamus Tinctorius L.)Under non Stress and Drought Stress Conditions

This study was conducted to determine drought tolerance genotypes with superiority in different stressed environments. To screening quantitative indices of drought tolerance, genotypes of safflower (Carthamus tinctorius L.) were tested in a complete randomized block design with three replications under two different water regimes (irrigated and rainfed). Significant positive correlatio...

متن کامل

study of the biochemical responses and enzymatic activity of gf677 (peach and almond hybrid) rootstock to in vitro salinity stress

introduction: salinity is the most significant abiotic factor limiting crop productivity and several physiological responses, including modification of ion balance, water status, mineral nutrition, stomatal behavior, photosynthetic efficiency and so on. the gf677 (prunuspersica×prunusamygdalus) is widelyusedas rootstock for peach and almond. it is mainly used as a rootstock because of its resis...

متن کامل

Physiological and Morphological Responses of Almond Cultivars under In Vitro Drought Stress

In this study, physiological and morphological responses of five almond cultivars to drought stress were investigated under in vitro conditions. Plantlets from five commercial almond cultivars (Supernova’, ‘Tuono’, ‘Sahand’, ‘Ferragnes ’ and ‘Shahroud 21’] were established in MS medium containing 0.5 mg per liter BAP and then subcultured  in MS proliferation medium containing 1 mg per liter BAP...

متن کامل

Evaluation of some drought tolerance genes expressions and enzymatic changes in sugar beet monogerm cultivars under drought stress conditions

Sugar beet is a strategic and industrial crop and important in sugar production. Drought is a limited factor in sugar beet yield. Base on this, an experiment carried out using a split-plot format in the randomized complete block design with 4 replications in 1394-5 at Chenaran area (Bahman Jan village). The main plot included three drop irrigation regimes: 7, 14 and 21 days interval as normal, ...

متن کامل

Investigation on the changes of some biochemical traits of almond genotypes leaves under drought stress on the GN15 rootstock

Abstract The selection of the appropriate almond cultivars (Prunus dulcis Mill.) in dry and semi-arid regions like Iran in order to produce the optimal product is the importance. In order to study tolerance to water deficit stress, One-year old plants five almond cultivars/ genotypes on the GN15, A factorial experiment was conducted with a RCD which included 3 irrigations factors, 5 genotype...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
به زراعی کشاورزی

جلد ۱۶، شماره ۳، صفحات ۷۰۷-۷۱۶

کلمات کلیدی
[ ' m e c h a n i s m s o f d r o u g h t s t r e s s t o l e r a n c e o f g f 6 7 7 r o o t s t o c k ' , ' p e a c h a n d a l m o n d h y b r i d ' , ' ( p r u n u s p e r s i c a × p r u n u s a m y g d a l u s ) w e r e s t u d i e d u n d e r i n v i t r o c o n d i t i o n s . p l a n t l e t s o f g f 6 7 7 r o o t s t o c k w e r e s u b c u l t u r e d i n t o t h e m u r a s h i g e a n d s k o o g ( m s ) s o l i d p r o l i f e r a t i o n m e d i u m c o n t a i n i n g 1 m g / l b a ( 6 ' , ' b e n z y l a d e n i n e ) a n d 0 . 1 m g / l n a a ( n a p h t h a l i n e a c e t i c a c i d ) i n f o u r d r o u g h t s t r e s s l e v e l s 0 ( c o n t r o l ) ' , 1 0 , ' 2 0 a n d 3 0 g / l p o l y e t h y l e n e g l y c o l 6 0 0 0 ( c o r r e s p o n d i n g t o o s m o t i c p o t e n t i a l s 0 ' , ' 0 . 2 ' , ' 0 . 4 a n d ' , ' 0 . 6 m p a ' , ' r e s p e c t i v e l y ) . a f t e r s i x w e e k s r e s u l t s s h o w e d t h a t i n d u c e d d r o u g h t s t r e s s h a d a s i g n i f i c a n t e f f e c t o n m e a s u r e d p a r a m e t e r s . b y i n c r e a s i n g d r o u g h t l e v e l s i n t h e c u l t u r e m e d i u m ' , ' a n t i o x i d a n t e n z y m e s a c t i v i t y ( c a t a l a s e a n d p e r o x i d a s e ) ' , ' t o t a l p r o t e i n c o n t e n t a n d p r o l i n e c o n t e n t s i g n i f i c a n t l y i n c r e a s e d ; m e a n w h i l e s o l u b l e s u g a r s h a d n o n ' , ' s i g n i f i c a n t i n c r e a s i n g i n t h e d i f f e r e n t l e v e l s o f d r o u g h t . a c c o r d i n g t o t h e r e s u l t s i t c a n b e c o n c l u d e d t h a t ' , ' t h e m o s t i m p o r t a n t m e c h a n i s m s o f d r o u g h t t o l e r a n c e o f g f 6 7 7 r o o t s t o c k u n d e r i n v i t r o c o n d i t i o n s a r e t h e u s e o f a n t i o x i d a n t d e f e n s e s y s t e m ' , ' i n c r e a s i n g p r o t e i n s y n t h e s i s ( e n h a n c i n g g e n e s e x p r e s s i o n ) a n d p r o l i n e a c c u m u l a t i o n a n d o s m o r e g u l a t i o n b y s o l u b l e s u g a r s h a d l e s s i m p o r t a n t . ' ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023